Lipidomics, originally a branch of metabolomics, has now evolved into an independent approach aimed at qualitatively and quantitatively characterizing lipids in biological systems. Its goal is to understand the complex molecular mechanisms underlying lipid remodeling in biological samples following diseases, therapeutic treatments, or nutritional interventions. Our services cover the entire process, from sample analysis to data processing and biological interpretation, as well as the setup of the entire experimental study, to support pharmaceutical, biochemical, and nutraceutical research.

1) Untargeted Lipidomic Analysis

Our lipidomic analysis package is applicable to various matrices, including plasma, cells, and tissues, depending on the request. It covers between 21 and 58 lipid subclasses to comprehensively explore lipid remodeling. Samples are extracted and processed using analytical techniques based on ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS), integrating ion mobility technology. The reporting delivery time depends on the sample size. A minimum of 3-5 replicates per condition is recommended.
Results: The report will include lipid identification through MS/MS and CCS spectral library comparison, as well as comparison with authentic standards. Semi-quantification in relative concentration will be performed using deuterated internal standards. Additionally, differential statistical analysis (univariate and multivariate), enrichment analysis, and pathway analysis will be provided. Upon request, data interpretation will be included to support the preparation of extended reports or assistance in writing papers or grant applications. Based on preliminary results and sample quality assessment, clients will be advised on whether to proceed or pause the analytical process to improve data quality.

2) Targeted Lipidomic Analysis

If a specific class of lipids is of interest, absolute quantitative analysis can be performed through on-demand packages for individual lipids or their combinations. The methodologies are based on liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) and/or gas chromatography coupled with mass spectrometry (GC-MS).

Results: The report will include lipid identification through comparison with authentic standards via retention times and MS/MS spectra, along with the molar values of the lipids of interest.
Upon request, differential statistical analysis (univariate and multivariate) will be provided. If required, data interpretation can be included to support the preparation of extended reports or assistance in writing papers or grant applications.